By Hadley Gavin
Accurate pipetting is an essential skill for any laboratory technician or researcher. Whether you are measuring precise volumes of liquid, diluting solutions, or transferring samples from one container to another, the accuracy and precision of your pipetting can have a profound impact on the success of your experiments.
Having a good handle on how to use a pipettor correctly is therefore critical for anyone who works in the lab. In this article we’ll discuss some techniques and strategies that will help you increase accuracy with a pipettor so that your results will be more reliable and reproducible.
Use the Right Pipettor for Each Task
Different types of pipettors are available for different tasks and volume ranges, so it is important to select the right one for your specific needs. For example, if you need to dispense small volumes of liquid, consider using a micropipette. Micropipettes have smaller diameters and shorter lengths than regular pipettes, making them ideal for handling small volumes.
On the other hand, if you need to dispense larger quantities of liquid, consider using a multi-channel or electronic pipettor. Multi-channel and electronic pipettors are designed for rapid dispensing of large volumes, so they can save time and increase accuracy in these cases.
Adopt the Right Technique
There are multiple techniques for pipetting, from basic single-handed use to more advanced multi-channel and reverse pipetting. It is important to find a technique that is comfortable for you, as this will help ensure accuracy.
Make sure that any new techniques you learn correctly, such as how to use a reverse pipettor, are properly practiced and perfected before using them for precise measurements.
Calibrate the Pipette Regularly
It is important to ensure your pipettor is always calibrated correctly in order to obtain accurate results. Depending on how often you use it, calibration should be done at least once every six months. Most pipettors have an indicator that will let you know when it is time for recalibration.
Always Use a Pipettor with High Precision
When selecting a pipettor, it is important to select one that has high precision and accuracy. Look for ones that have calibration certificates or ISO certifications from independent testing laboratories to ensure their accuracy.
Also, using digital pipettors can help increase accuracy by allowing you to automate the dispense process and set precise parameters for each dispensing step, such as volume and speed.
Never Overfill Your Pipettor
Overfilling a pipettor can lead to errors in dispense volumes, so it is important to always check that the liquid level is below the maximum fill line before using it. Additionally, try to avoid over-saturating the tips with liquid, as this can make them difficult to remove and lead to inaccurate measurements.
Clean Your Pipettor After Each Use
Pipettors should be cleaned and maintained regularly to ensure they are working properly and accurately. Regular cleaning will help to remove any contaminants and residue that might affect the performance of the pipettor. It is also important to check for any signs of wear and tear, such as cracks or chips in the tip, as these can lead to inaccurate measurements.
Additionally, make sure to always use new tips with each dispense cycle to prevent cross-contamination and maintain accuracy.
Takeaway
Using these tips, you can increase the accuracy of your pipettor and ensure that your measurements are precise and reliable. Remember to select the right pipettor for each task, use one with high precision, never overfill it, and clean it after each use. By following these steps, you can make sure that your results are accurate and reliable.
